| 1 | Academy of Internal Troops of Ukraine | Kharkiv | 1931 | — |
| 2 | Universidad Nacional de Construcción Naval Almirante Makárov | Mykolayiv | 1920 | — |
| 3 | Alfred Nobel University | Dnipro | 1993 | — |
| 4 | American-Ukrainian School of Computer Sciences and Technologies | — | 2004 | — |
| 5 | American University of Kyiv | Kyiv | — | — |
| 6 | Berdyan University of Management and Business | — | 1992 | — |
| 7 | Bogdan Khmelnitsky Melitopol State Pedagogical University | Melitopol | 1923 | — |
| 8 | Universidad Nacional Médica Bogolomets | Kyiv | 1841 | — |
| 9 | Borys Grinchenko Kyiv Metropolitan University | Kyiv | 1874 | — |
| 10 | Bukovinian State Medical University | Chernivtsi | 1944 | — |
| 11 | Bukovinian University | — | 1995 | — |
| 12 | Cherkasy National University | Cherkasy | 1921 | — |
| 13 | Cherkasy State Technological University | Cherkasy | 1960 | — |
| 14 | Chernihiv Polytechnic National University | Chernihiv | 1960 | — |
| 15 | Classic Private University | Zaporizhzhya | 1992 | — |
| 16 | Danylo Halytsky Lviv National Medical University | Lviv | 1784 | — |
| 17 | Dnipro Institute of Infrastructure and Transport | — | 1930 | — |
| 18 | Dnipropetrovsk Regional Institute of Public Administration | Dnipro | 1993 | — |
| 19 | Dnipro State Agrarian University | Dnipro | 1922 | — |
| 20 | Dnipro State Financial Academy | Dnipro | 1977 | — |
| 21 | Dnipro State Medical University | Dnipro | 1801 | — |
| 22 | Dnipro State University of Internal Affairs | Dnipro | 1966 | — |
| 23 | Dniprovsky State Technical University | Kamyanske | 1920 | — |
| 24 | Donbas National Academy of Civil Engineering and Architecture | Kramatorsk | 1972 | — |
| 25 | Donbas State Engineering Academy | Kramatorsk | 1952 | — |
| 26 | Donbas State Pedagogical University | Kramatorsk | 1939 | — |
| 27 | Donbas State Technical University | Alchevsk | 1957 | — |
| 28 | Donetsk Christian University | Donetsk | 1991 | — |
| 29 | Donetsk National Medical University | Donetsk | 1930 | — |
| 30 | Universidad Técnica Nacional de Donetsk | Drohobych | 1921 | — |
| 31 | Donetsk National University of Economics and Trade | Kryvyy Rih | 1920 | — |
| 32 | Donetsk Railway Transport Institute | Donetsk | 1967 | — |
| 33 | Donetsk State University of Internal Affairs | — | 1961 | — |
| 34 | Drohobych Ivan Franko State Pedagogical University | Drohobych | 1998 | — |
| 35 | Drohobych Petroleum and Gas College | — | 1945 | — |
| 36 | East European Slavic University | — | — | — |
| 37 | Universidad Nacional Volodymyr Dahl de Ucrania Oriental | Luhansk | 1920 | — |
| 38 | Engineering Institute of Zaporizhzhia National University | Zaporizhzhya | 1959 | — |
| 39 | Evangel Theological Seminary | Kyiv | 2000 | — |
| 40 | First Ukrainian maritime institute | — | 2005 | — |
| 41 | Academia Nacional de las Fuerzas Terrestres Hetman Petro Sahaidachnyi | Lviv | 1899 | — |
| 42 | Hlukhiv National Pedagogical University of Oleksandr Dovzhenko | Hlukhiv | 1874 | — |
| 43 | Horlivka Institute for Foreign Languages | Horlivka | 1949 | — |
| 44 | Universidad Nacional de Pedagogía H. S. Skovorodá de Járkov | Kharkiv | 1804 | — |
| 45 | Instituto Politécnico de Kiev | Kyiv | 1898 | — |
| 46 | Information-diagnostic Systems Institute | — | — | — |
| 47 | International Christian University – Kiev | — | 1992 | — |
| 48 | International European University | Kyiv | 1999 | — |
| 49 | International Institute of Business | — | 1993 | — |
| 50 | International Management Institute | Kyiv | 1989 | — |
| 51 | International Solomon University | Kyiv | 1991 | — |
| 52 | Academia Interregional de Gestión de Personal | Kyiv | 1989 | — |
| 53 | Universidad Nacional de Medicina de Ivano-Frankivsk | Ivano-Frankivsk | 1945 | — |
| 54 | Ivano-Frankivsk National Technical University of Oil and Gas | — | 1967 | — |
| 55 | Ivano-Frankivsk Theological Academy of Greek-Catholic Church | Ivano-Frankivsk | 1991 | — |
| 56 | Universidad Estatal de Medicina de Ternopil | Ternopil | 1957 | — |
| 57 | Izmail State University of Humanities | Izmail | 1940 | — |
| 58 | Jewish University of Odesa | — | 2003 | — |
| 59 | Kamianets-Podilskyi Ivan Ohiienko National University | Kamianets-Podilskyi | 1918 | — |
| 60 | Colegio Kharkiv de ingeniería textil y diseño | — | 1935 | — |
| 61 | Academia Médica de Educación Superior de Járkov | Horlivka | 1923 | — |
| 62 | Kharkiv National Agrarian University named after V.V. Dokuchayev | Saltivka | 1816 | — |
| 63 | Kharkiv National Automobile and Highway University | Kharkiv | 1930 | — |
| 64 | Kharkiv National Medical University | Kharkiv | 1920 | — |
| 65 | Kharkiv National University of Construction and Architecture | Kharkiv | 1930 | — |
| 66 | Universidad Nacional de Radioelectrónica de Járkov | Kharkiv | 1930 | — |
| 67 | Universidad Nacional Técnica de Agricultura Petro Vasylenko | Horlivka | 1930 | — |
| 68 | Instituto Politécnico de Járkov | Kharkiv | 1885 | — |
| 69 | Universidad Estatal de Tecnología Alimentaria y Comercio de Járkov | Kharkiv | 1967 | — |
| 70 | Kharkiv University of Humanities “People’s Ukrainian Academy” | Kharkiv | 1991 | — |
| 71 | Kharkov Institute of National Economy | Kharkiv | 1920 | — |
| 72 | Universidad Nacional Técnica de Jerson | Kherson | 1959 | — |
| 73 | Kherson State Maritime Academy | Kherson | 1834 | — |
| 74 | Universidad Nacional de Jmelnitsky | Khmelnytskyi | 1962 | — |
| 75 | KhVVKIU | Kharkiv | 1941 | — |
| 76 | King Danyo University | Ivano-Frankivsk | 1997 | — |
| 77 | Kremenchuk Mykhailo Ostrohradskyi National University | Kremenchuk | 1960 | — |
| 78 | Kryvyi Rih National University | Kryvyy Rih | 1922 | — |
| 79 | Kyiv Academic University | Svyatoshyn | — | — |
| 80 | Kyiv International University | Svyatoshyn | 1994 | — |
| 81 | Kyiv Medical University | Kyiv | 1992 | — |
| 82 | Kyiv National Economic University named after Vadym Hetman | — | 1906 | — |
| 83 | Universidad Nacional de Teatro | Kyiv | 1904 | — |
| 84 | Universidad Nacional de Kiev de Construcción y Arquitectura | Kyiv | 1930 | — |
| 85 | Universidad Nacional de Cultura y Artes de Kiev | — | 1956 | — |
| 86 | Kyiv National University of Technologies and Design | Kyiv | 1930 | — |
| 87 | Kyiv University of Law | Svyatoshyn | 1995 | — |
| 88 | Kyiv University of Management and Entrepreneurship | Kyiv | 2011 | — |
| 89 | Kyiv University of Tourism, Economics and Law | Kyiv | 1995 | — |
| 90 | Lesya Ukrainka Volyn National University | Lutsk | 1940 | — |
| 91 | Lugansk National Agrarian University | Kharkiv | 1921 | — |
| 92 | Luhansk State University of Internal Affairs | Luhansk | 1993 | — |
| 93 | Lutsk National Technical University | Lutsk | 1966 | — |
| 94 | Lviv National Academy of Arts | Lviv | 1946 | — |
| 95 | Lviv National Environmental University | Lviv | 1856 | — |
| 96 | Universidad Politécnica Nacional de Leópolis | Lviv | 1844 | — |
| 97 | Lviv State University of Internal Affairs | Lviv | 1939 | — |
| 98 | Lviv State University of Life Safety | Lviv | 1947 | — |
| 99 | Lviv State University of Physical Culture | Lviv | 1946 | — |
| 100 | Lviv University of Trade and Economics | Lviv | 1816 | — |
